Release of NPY in pig pancreas: Dual parasympathetic and sympathetic regulation

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6896350

Sheikh, S P; Holst, J J; Skak-Nielsen, T; Knigge, U; Warberg, J; Theodorsson-Norheim, E; Hoekfelt, T; Lundberg, J M; Schwartz, T W ^[1]

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1. Univ. of Copenhagen (Denmark) Karolinska Institutet, Stockholm (Sweden)

Several lines of evidence have connected neuropeptide Y (NPY), a 36-residue polypeptide, to the sympathetic division of the autonomic nervous system. The authors studied the localization, the molecular characteristics, and the release of NPY and norepinephrine (NE) in the porcine pancreas. Immunohistochemical investigations revealed that NPY nerves around blood vessels were likely to be of adrenergic nature, whereas NPY-immunoreactive fibers close to exocrine and endocrine cells may originate from local ganglia also containing VIP (vasoactive intestinal peptide) and PHI (peptide histidine isoleucine). Electrical stimulation of the splanchnic nerve supply to the isolated perfused pig pancreas resulted in a corelease of NPY and NE into the venous effluent. Stimulation of the vagal nerves caused a sevenfold larger release of NPY without affecting the NE secretion. Characterization of the NPY immunoreactivity in the pancreatic tissue and in the venous effluent by gel filtration, high-performance liquid chromatography, and isoelectric focusing shoed that the immunoreactive NPY was indistinguishable from synthetic porcine NPY. It is concluded that, although NPY is associated with sympathetic perivascular neurons, the majority of the pancreatic NPY-containing nerve fibers are likely to belong to the parasympathetic division of the autonomic nervous system.

• OSTI ID: 6896350
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:1; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOELECTRICITY
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
FLUORESCENCE
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LUMINESCENCE
MAMMALS
NERVES
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PERFUSED TISSUES
PHYSIOLOGY
POLYPEPTIDES
PROTEINS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
SUBCELLULAR DISTRIBUTION
SWINE
SYMPATHOMIMETICS
TISSUES
VERTEBRATES