Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.12.4372· OSTI ID:5444050

Kamb, A; Weir, M; Rudy, B; Varmus, H; Kenyon, C ^[1]

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1. Univ. of California, San Francisco (USA)

The study of gene family members has been aided by the isolation of related genes on the basis of DNA homology. The authors have adapted the polymerase chain reaction to screen animal genomes very rapidly and reliably for likely gene family members. Using conserved amino acid sequences to design degenerate oligonucleotide primers, they have shown that the genome of the nematode Caenorhabditis elegans contains sequences homologous to many Drosophila genes involved in pattern formation, including the segment polarity gene wingless (vertebrate int-1), and homeobox sequences characteristic of the Antennapedia, engrailed, and paired families. In addition, they have used this method to show that C. elegans contains at least five different sequences homologous to genes in the tyrosine kinase family. Lastly, they have isolated six potassium channel sequences from humans, a result that validates the utility of the method with large genomes and suggests that human potassium channel gene diversity may be extensive.

• OSTI ID: 5444050
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:12; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACID SEQUENCE
ANIMALS
ARTHROPODS
ASCHELMINTHES
BIOLOGICAL EVOLUTION
DIPTERA
DNA POLYMERASES
DNA SEQUENCING
DROSOPHILA
ENZYMES
FLIES
FRUIT FLIES
GENE AMPLIFICATION
GENES
HELMINTHS
HYDROXYLASES
INSECTS
INVERTEBRATES
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MOLECULAR STRUCTURE
NEMATODES
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POTASSIUM COMPOUNDS
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
TYROSINASE
VERTEBRATES