Molecular cloning of the human casein kinase II. alpha. subunit

Journal Article · Tue May 02 00:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00435a066· OSTI ID:5443587

Meisner, H; Heller-Harrison, R; Buxton, J; Czech, M P ^[1]

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1. Univ. of Massachusetts Medical School, Worcester (USA)

A human cDNA encoding the {alpha} subunit of casein kinase II and a partial cDNA encoding the rat homologue were isolated by using a Drosophila casein kinase II cDNA probe. The 2.2-kb human cDNA contains a 1.2-kb open reading frame, 150 nucleotides of 5{prime} leader, and 850 nucleotides of 3{prime} noncoding region. Except for the first 7 deduced amino acids that are missing in the rat cDNA, the 328 amino acids beginning with the amino terminus are identical between human and rat. The Drosophila enzyme sequence is 90% identical with the human casein kinase II sequence, and there is only a single amino acid difference between the published partial bovine sequence and the human sequence. In addition, the C-terminus of the human cDNA has an extra 53 amino acids not present in Drosophila. Northern analysis of rat and human RNA showed predominant bands of 5.5, 3.1, and 1.8 kb. In rat tissues, brain and spleen had the highest levels of casein kinase II {alpha} subunit specific RNA, while skeletal muscle showed the lowest. Southern analysis of human cultured cell and tissue genomic DNA using the full-length cDNA probe revealed two bands with restriction enzymes that have no recognition sites within the cDNA and three to six bands with enzymes having single internal sites. These results are consistent with the possibility that two genes encode the {alpha} subunits.

• OSTI ID: 5443587
• Journal Information: Biochemistry; (USA), Vol. 28:9; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOTRANSFERASES
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
BIOLOGICAL EVOLUTION
CASEIN
DNA-CLONING
DROSOPHILA
GENES
MAN
PHOSPHORUS 32
RATS
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
DAYS LIVING RADIOISOTOPES
DIPTERA
DNA
DNA HYBRIDIZATION
ENZYMES
FLIES
FRUIT FLIES
HYBRIDIZATION
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques