Complete amino acid sequence and homologies of human erythrocyte membrane protein band 4. 2

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.2.613· OSTI ID:6700166

Korsgren, C; Lambert, S; Cohen, C M ^[1]; Lawler, J ^[2]; Speicher, D ^[3]

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1. St. Elizabeth's Hospital, Boston, MA (USA) Tufts Univ. School of Medicine, Boston, MA (USA)
2. Brigham and Women's Hospital and Harvard Medical School, Boston, MA (USA)
3. Wistar Institute, Philadelphia, PA (USA)

The complete amino acid sequence for human erythrocyte band 4.2 has been derived from the nucleotide sequence of a full-length 2.35-kilobase (kb) cDNA. The 2.35-kb cDNA was isolated from a human reticulocyte cDNA library made in the expression vector {lambda}gt11. Of the 2348 base pairs (bp), 2073 bp encode 691 amino acids representing 76.9 kDa. RNA blot analysis of human reticulocyte total RNA gives a message size for band 4.2 of 2.4 kb. The amino acid sequence of band 4.2 has homology with two closely related Ca{sup 2+}-dependent crosslinking proteins, guinea pig liver transglutaminase and the a subunit of human coagulation factor XIII, transglutaminase that forms intermolecular {gamma}-glutamyl-{epsilon}-lysine bonds between fibrin molecules. The region of greatest identity includes a 49-amino acid stretch of band 4.2, which is 69% and 51% identical with guinea pig liver transglutaminase and the a subunit of factor XIII, respectively, within the regions that contain the active sites of these enzymes. Significantly, within the five contiguous consensus residues of the transglutaminase active site, Gly-Gln-Cys-Trp-Val, band 4.2 has an alanine substituted for cysteine. Consistent with this active site substitution, erythrocyte membranes or inside-out vesicles, which contain band 4.2, show no evidence of transglutaminase activity by two types of in vitro assay.

• OSTI ID: 6700166
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:2; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
DOMESTIC ANIMALS
ENZYMES
ERYTHROCYTES
GLANDS
GLUTAMIC ACID
GLYCINE
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER
LYSINE
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RETICULOCYTES
RNA
STRUCTURAL CHEMICAL ANALYSIS
SWINE
TRANSFERASES
VERTEBRATES