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Increased radiation-induced transformation in C3H/10T1/2 cells after transfer of an exogenous c-myc gene

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
C3H/10T 1/2 cells were infected with a retroviral vector expressing a mouse c-myc oncogene and a drug-selection marker. The resulting cells, morphologically indistinguishable from C3H/10T l/1, displayed a greatly enhanced sensitivity to neoplastic transformation by ionizing radiation or by a chemical carcinogen. Constitutive expression of myc therefore appears to synergize with an initial carcinogenic event, providing a function analogous to a subsequent event that apparently is required for the neoplastic transformation of these cells. This cell system should prove useful in exploring early stages in radiation-induced transformation.
Research Organization:
Cornell Univ. Graduate School of Medical Sciences, New York, NY
OSTI ID:
5441715
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:12; ISSN PNASA
Country of Publication:
United States
Language:
English

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