Synthesis of subgenomic mRNA's of mouse hepatitis virus is initiated independently: evidence from UV transcription mapping

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · J. Virol.; (United States)

OSTI ID:5440910

Jacobs, L; Spaan, W J; Horzinek, M C; van der Zeijst, B A

The target sizes of the templates for the synthesis of the genome-sized RNA and the six subgenomic RNAs found in cells infected with mouse hepatitis virus strain A59 were determined by UV transcription mapping. Infected Sac(-) cells were irradiated at 6 h postinfection, the time when virus-specific RNA synthesis starts to increase exponentially. The effect of increasing UV doses on the synthesis of the individual RNAs was determined by quantitation of these RNAs after separation by agarose gel electrophoresis. The UV target sizes calculated for the templates were almost identical to the physical sizes of the RNAs. The results of these experiments seem to exclude the possibility that the subgenomic RNAs are processed or spliced from a common precursor. The data are consistent with independent initiation of transcription on a genome-sized, negative-stranded template or on smaller templates.

• OSTI ID: 5440910
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 39:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CHEMISTRY
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
INFECTIOUS DISEASES
INFECTIOUS HEPATITIS
MESSENGER-RNA
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PRECURSOR
RADIATION EFFECTS
RADIATIONS
RNA
SYNTHESIS
TRANSCRIPTION
ULTRAVIOLET RADIATION
VIRAL DISEASES
VIRUSES