Heterogeneous nuclear ribonuclear protein K interacts with Sindbis virus nonstructural proteins and viral subgenomic mRNA

Journal Article · Wed Oct 10 04:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2007.05.008· OSTI ID:21077982

Burnham, Andrew J; Gong, Lei ^[1]

---

1. Department of Biology, Indiana University, 1001 E. Third Street, Bloomington, IN 47405 (United States)

Alphaviruses are a group of arthropod-borne human and animal pathogens that can cause epidemics of significant public health and economic consequence. Alphavirus RNA synthesis requires four virally encoded nonstructural proteins and probably a number of cellular proteins. Using comparative two-dimensional electrophoresis we were able to identify proteins enriched in cytoplasmic membrane fractions containing viral RNA synthetic complexes following infection with Sindbis virus. Our studies demonstrated the following: (i) the host protein hnRNP K is enriched in cytoplasmic membrane fractions following Sindbis virus infection, (ii) viral nonstructural proteins co-immunoprecipitate with hnRNP K, (iii) nsP2 and hnRNP K co-localize in the cytoplasm of Sindbis virus infected cells, (iv) Sindbis virus subgenomic mRNA, but not genomic RNA co-immunoprecipitates with hnRNP K, (v) viral RNA does not appear to be required for the interaction of hnRNP K with the nonstructural proteins. Potential functions of hnRNP K during virus replication are discussed.

• OSTI ID: 21077982
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 367; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ARTHROPODS
CELL MEMBRANES
CYTOPLASM
PATHOGENS
PROTEINS
PUBLIC HEALTH
RNA
VIRUSES