The nuclear protein encoded by the Drosophila neurogenic gene mastermind is widely expressed and associates with specific chromosomal regions

Journal Article · Sat Jun 01 04:00:00 EDT 1996 · Genetics

OSTI ID:543024

Bettler, D; Pearson, S; Yedvobnick, B ^[1]

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1. Emory Univ., Atlanta, GA (United States)

The Drosophila neurogenic loci encode a diverse group of proteins that comprise an inhibitory signal transduction pathway. The pathway is used throughout development in numerous contexts. We have examined the distribution of the neurogenic locus mastermind protein (Mam). Mam is expressed through all germlayers during early embryogenesis, including ectodermal precursors to both neuroblasts and epidermoblasts. Mam is subsequently down-regulated within the nervous system and then reexpressed. It persists in the nervous system through late embryogenesis and postembryonically. Mam is ubiquitously expressed in wing and leg imaginal discs and is not down-regulated in sensory organ precursor cells of the wing margin or notum. In the eye disc, Mam shows most prominent expression posterior to the morphogenetic furrow. Expression of the protein during oogenesis appears limited to follicle cells. Immunohistochemical detection of Mam on polytene chromosomes revealed binding at >100 sites. Chromosome colocalization studies with RNA polymerase and the groucho corepressor protein implicate Mam in transcriptional regulation. 94 refs., 8 figs., 1 tab.

• OSTI ID: 543024
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 2 Vol. 143; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMES
DNA SEQUENCING
DROSOPHILA
GENE REGULATION
GENES
GENETIC MAPPING
IMMUNOASSAY
NERVOUS SYSTEM
ONTOGENESIS
OOGENESIS
PROTEINS
RNA POLYMERASES
TRANSCRIPTION