Selective lymphoid irradiation. I. An approach to transplantation. [Rats]

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · Surgery; (United States)

OSTI ID:5429707

Hardy, M A; Fawwaz, R A; Oluwole, S; Todd, G; Nowygrod, R; Reemtsma, K

The kinetics, distribution, and radiobiologic effects of palladium (Pd)-109-hematoporphyrin were determined in the rat. In addition, we studied the effect on rat heart allograft survival of Pd-109-hematoporphyrin, with and without antilymphocyte serum (ALS). A single sublethal dose of Pd-109-hematoporphyrin (up to 36 muCi/kg) resulted in the following: predominant concentration in lymphoid tissue and proximal bone marrow, complete central and proximal bone marrow ablation with preservation of distal bone marrow, massive depletion of lymphocytes from lymph nodes and spleen, an 80% reduction in peripheral blood lymphocytes which was completed by the addition of ALS, full recovery of lymphoid tissue and blood cellularity within 60 days of administration of radionuclide, and a 100% animal survival rate. This method of selective lymphoid irradiation (SLI) prolongs indefinitely Fisher cardiac allografts in Lewis recipients and significantly prolongs cardiac allograft survival across major histocompatibility barriers (ACI to Lewis or to Fisher). Specific tolerance to donor strains was demonstrated by the acceptance of Fisher skin by Lewis recipients carrying 150-day-old Fisher hearts. Third party (ACI) skin allografts were rapidly rejected by the same animals. Further studies of SLI in larger animals are required to determine the optimal safe dose of SLI in man.

• Research Organization: Columbia Univ. Coll. of Physicians and Surgeons, New York
• OSTI ID: 5429707
• Journal Information: Surgery; (United States), Journal Name: Surgery; (United States) Vol. 86:2; ISSN SURGA
• Country of Publication: United States
• Language: English

Related Subjects

550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
560152* -- Radiation Effects on Animals-- Animals
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DRUGS
EVEN-ODD NUCLEI
GRAFT-HOST REACTION
GRAFTS
HEART
HEMATOPORPHYRINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
IMMUNITY
INTERMEDIATE MASS NUCLEI
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PALLADIUM 109
PALLADIUM ISOTOPES
PIGMENTS
PORPHYRINS
RADIATION EFFECTS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
SKIN
SOMATIC CELLS
SUBLETHAL IRRADIATION
TRANSPLANTS