Selective lymphoid irradiation. VI. Effect of palladium-109-labeled lymphocytes on rat cardiac allograft survival

Journal Article · Thu Aug 01 00:00:00 EDT 1985 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198508000-00007· OSTI ID:5095485

Oluwole, S; Fawwaz, R; Kuromoto, N; Reemtsma, K; Hardy, M A

The authors have previously shown that pretransplant treatment with palladium-109 hematoporphyrin (Pd-H) and two small doses of antilymphocyte globulin (ALG) leads to donor-specific permanent acceptance of cardiac allografts in weakly histoincompatible rat combinations and significant prolongation of hearts in the ACI-to-Lewis strains. Pd-H also concentrates in nonlymphoid organs to a significant degree that is undesirable, so the authors have examined, the efficacy of Pd-109 attached to lymphocytes in producing selective lymphoid irradiation and compared it with the immunosuppressive effect of Pd-H. Adoptive transfer of syngeneic lymphocytes labeled with palladium-109 (Pd-L) led to significant concentration of radioactivity in the peripheral lymphoid organs relative to the bone marrow (BM), intestine, thymus, and endocrine organs. The concentration of radioactivity in the spleen relative to BM and intestinal mucosa was 23:1 and 58:1, respectively. The immunosuppressive efficacy of a suboptimal dose of 3 mCi (one-third of the dose used in earlier reports with Pd-H) administered via Pd-L (6 X 10(9) cells) at 4 days combined with 5 mg ALG at 2 days and 1 day prior to transplantation was compared in its effect on cardiac allografts with a similar dose of Pd-H (3 mCi) combined with ALG. The mean survival time (MST) of 6.5 +/- 0.4 days in untreated recipients was moderately prolonged to 14.6 +/- 3.0 days by Pd-H and ALG, and was not significantly different from an MST of 14.1 +/- 0.3 days achieved with 2 doses of ALG alone. Pretransplant treatment of Lewis rats with Pd-L and ALG produced a significant prolongation of ACI heart allografts to 30.5 +/- 3.1 days (P less than 0.001).

• Research Organization: Univ. of Ife, Nigeria
• OSTI ID: 5095485
• Journal Information: Transplantation; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
LABELLING
TRANSPLANTS
SURVIVAL TIME
BONE MARROW
COMPARATIVE EVALUATIONS
ENDOCRINE GLANDS
GLOBULINS
HEART
HEMATOPORPHYRINS
IMMUNOSUPPRESSION
INTESTINES
IRRADIATION
PALLADIUM 109
RADIOSENSITIVITY EFFECTS
RATS
THYMUS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
GLANDS
HEMATOPOIETIC SYSTEM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PALLADIUM ISOTOPES
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
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