Renal catabolism of /sup 125/I-glicentin

Journal Article · Thu May 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:5429162

Lopez-Novoa, J M; Santos, J C; Villamediana, L M; Garrote, F J; Thim, L; Moody, A J; Valverde, I

The renal catabolism of /sup 125/I-glicentin has been studied in vivo by the disappearance of this peptide from the plasma of bilaterally nephrectomized, ureteral-ligated, or normal rats and by using tubular microinfusion techniques. In addition the catabolism of glicentin by the isolated, perfused kidney has been studied. Results from in vivo studies demonstrated that half-disappearance time was lower in control (59.5 +/- 1.8 min) than in bilaterally nephrectomized rats (97.2 +/- 2.6 min), and this value was significantly higher than that of ureteral-ligated animals (83.2 +/- 1.1 min, P less than 0.005). Microinfusion experiments revealed that when /sup 125/I-glicentin was injected into the proximal tubule, no trichloroacetic-precipitable radioactivity was recovered in the urine, whereas most of inulin injected was recovered. By contrast most of the /sup 125/I-glicentin injected into the distal tubule was recovered in the urine. In isolated kidney experiments, organ clearance rate of /sup 125/I-glicentin averaged 0.88 +/- 0.10 ml/min, a value significantly higher than that of glomerular filtration rate (0.72 +/- 0.06 ml/min, P less than 0.005, paired data), and both parameters showed a close linear relationship (r = 0.90). Urinary clearance of glicentin was negligible. These results demonstrate that the kidney plays a major role in the catabolism of glicentin, mainly by glomerular filtration and tubular catabolism. The site of tubular catabolism appears to be the proximal tubule. Peritubular uptake was minimal.

• Research Organization: Fundacion Jimenez Diaz, Madrid, Spain
• OSTI ID: 5429162
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CATABOLISM
CLEARANCE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
GLUCAGON
HORMONES
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
MAMMALS
MATERIALS
MEDICINE
METABOLISM
NEPHRECTOMY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PERFUSED ORGANS
POLYPEPTIDES
PRECIPITATION
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RENAL CLEARANCE
RODENTS
SEPARATION PROCESSES
SURGERY
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES