Effects of methylprednisolone on concanavalin A-induced human lymphocyte blastogenesis: a comparative analysis by flow cytometry, volume determination and /sup 3/H-thymidine incorporation
Journal Article
·
· Immunopharmacology; (United States)
The inhibition of concanavalin A-induced human peripheral blood lymphocyte blastogenesis by methylprednisolone (MP) was studied by using flow cytometry and tritiated thymidine (/sup 3/H-TdR) incorporation. Flow cytometric determinations of volume, low angle forward light scatter, and nucleic acid showed MP to be a potent inhibitor of blastogenesis. The effects were concentration-dependent and correlated with /sup 3/H-TdR uptake. By using the single cell analytic capability of flow cytometry, the target stages of the cell cycle where MP affects lymphocyte activation were determined. Evidence is presented that steroids can block both early and late phases of this process.
- Research Organization:
- Department of Immunology Research, Lilly Research Laboratories, Indianapolis, Indiana
- OSTI ID:
- 5428989
- Journal Information:
- Immunopharmacology; (United States), Journal Name: Immunopharmacology; (United States) Vol. 6:2; ISSN IMMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CELL FLOW SYSTEMS
CONCANAVALIN
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DNA REPLICATION
GLUCOCORTICOIDS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
NUCLEIC ACID REPLICATION
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREDNISONE
PREGNANES
PRIMATES
PYRIMIDINES
SOMATIC CELLS
STEROID HORMONES
STEROIDS
STIMULATION
THYMINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
VERTEBRATES
VOLUME
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CELL FLOW SYSTEMS
CONCANAVALIN
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DNA REPLICATION
GLUCOCORTICOIDS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
NUCLEIC ACID REPLICATION
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREDNISONE
PREGNANES
PRIMATES
PYRIMIDINES
SOMATIC CELLS
STEROID HORMONES
STEROIDS
STIMULATION
THYMINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
VERTEBRATES
VOLUME