Modulation of intracellular glutathione concentrations alters lymphocyte activation and proliferation

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90305-3· OSTI ID:6528482

Fidelus, R K; Tsan, Minfu ^[1]; Ginouves, P; Lawrence, D ^[2]

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1. Veterans Administration Medical Center, Albany, NY (USA) Albany Medical College of Union Univ., NY (USA)
2. Albany Medical College of Union Univ., NY (USA)

Glutathione (GSH) has been implicated in lymphocyte activation and differentiation, as well as in protection from radiation damage. Since ({sup 3}H)thymidine (({sup 3}H)TdR) at high concentrations in the nucleus causes radiation damage to the cells, it is important to rule out the possibility that changes in ({sup 3}H)TdR uptake by mitogen-activated lymphocytes are not caused by {sup 3}H-induced cell injury following alterations in intracellular GSH concentration. In this study, flow-cytometric analysis of cell cycle was used to measure lymphocyte activation. Enhancement of intracellular GSH concentrations in lymphocytes with 2-oxothiazolidine-4-carboxylate or 2-mercaptoethanol augments mitogen-induced lymphocyte activation, and proliferation, while suppression of intracellular GSH levels by buthionine sulfoximine inhibits the progression of cellular proliferation-but not activation, as measured by flow cytometry. There was a linear relationship between intracellular GSH concentration and conA-activated cells by flow cytometry and between GSH concentration and ({sup 3}H)TdR incorporation as measured at 24 h. The authors conclude that alterations of intracellular GSH concentrations may be one way to modulate lymphocyte activation and differentiation.

• OSTI ID: 6528482
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 170:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
CELL FLOW SYSTEMS
CELL PROLIFERATION
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GLUTATHIONE
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RADIOPROTECTIVE SUBSTANCES
RIBOSIDES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS