Chronic cigarette smoke exposure adversely alters /sup 14/C-arachidonic acid metabolism in rat lungs, aortas and platelets

Journal Article · Mon Aug 08 00:00:00 EDT 1983 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(83)90133-9· OSTI ID:5426958

Lubawy, W C; Valentovic, M A; Atkinson, J E; Gairola, G C

Male rats were exposed to freshly generated cigarette smoke once daily, 5 times a week for 10 weeks. Inhalation of smoke was verified by elevated carboxyhemoglobin in blood sampled immediately after smoke exposure and by increased lung aryl hydrocarbon hydroxylase activity 24 hours after the last smoke exposure. Aortic rings isolated from smoke-exposed rats synthesized less prostacyclin (PGI2) from /sup 14/C-arachidonic acid than rings from sham rats. Platelets from smoke-exposed rats synthesized more thromboxane (TXA2) from /sup 14/C-arachidonic acid than platelets from room controls but not those from sham rats. Lung microsomes from smoke-exposed rats synthesized more TXA2 and had a lower PGI2/TXA2 ratio than lung microsomes from room controls and shams. It is concluded that chronic cigarette smoke exposure alters arachidonic acid metabolism in aortas, platelets and lungs in a manner resulting in decreased PGI2 and increased TXA2, thereby creating a condition favoring platelet aggregation and a variety of cardiovascular diseases.

• Research Organization: College of Pharmacy, Graduate Center for Toxicology, University of Kentucky, Lexington
• OSTI ID: 5426958
• Journal Information: Life Sci.; (United States), Vol. 33:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
METABOLISM
CARBOXYHEMOGLOBIN
QUANTITATIVE CHEMICAL ANALYSIS
PROSTAGLANDINS
BIOSYNTHESIS
TOBACCO SMOKES
BIOLOGICAL EFFECTS
AORTA
BLOOD PLATELETS
CARBON 14 COMPOUNDS
LUNGS
METABOLIC ACTIVATION
MIXED-FUNCTION OXIDASES
RATS
STIMULATION
TRACER TECHNIQUES
AEROSOLS
ANIMALS
ARTERIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
COLLOIDS
DISPERSIONS
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
SYNTHESIS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques