Reduced cytotoxic responses with normal CTL precursor numbers in tolerant mice

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5416283

La Rosa, F G; Smilek, D; Ammons, J T; Talmage, D W

Balb/c (H-2d) thyroid lobes were cultured for 48 hours in 2.5 atmospheres of O/sub 2/ and inserted under the kidney capsule of C57B1/6 (H-2b) mice. 36 out of 52 grafts survived for more than 30 weeks. Unlike freshly grafted mice long term recipients failed to reject the original grafts or a new transplant following the injection of donor spleen cells. Cytotoxic T lymphocytes precursor (CTLp) frequency was found to be in the same range (1/500 to 1/1000 spleen cells) in tolerant and normal mice. Nevertheless CTL responses were reduced both in vivo and in vitro. CTL responses in vivo were measured by injecting live P815 tumor cells (H-2d) i.p. into primed tolerant and normal C57B1/6 mice. The peak of specific /sup 51/Cr release (E:T = 80:1) of 9 normal mice was 39 +/- 7% on day 3, while the peak response of 11 tolerant mice was 12 +/- 2% on day 5. CTL responess measured in vitro were less strinkingly reduced than the in vivo responses. However, the in vitro responses were reduced in different experiments by either (1) reduced specific /sup 51/Cr release at the same E:T ratio or (2) reduced cell number at the end of the culture. Further evidence that the in vitro CTL responses of tolerant mice were suppressed was found in their restoration to normal levels by the addition of ConA supernatant to the culture media.

• Research Organization: Univ. of Colorado HSC, Denver
• OSTI ID: 5416283
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONCANAVALIN
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GRAFT-HOST REACTION
GRAFTS
HEMAGGLUTININS
IMMUNE REACTIONS
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LECTINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
ORGANS
PRECURSOR
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYROID
TRACER TECHNIQUES
TRANSPLANTS
TUMOR CELLS
VERTEBRATES