Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Induction of TNP-specific cytotoxic T lymphocyte memory in vivo in the absence of T helper cell activity

Journal Article · · J. Immunol.; (United States)
OSTI ID:5005742
; ;
The question of whether TH cells are required for the priming of CTL precursors (CTLp) in vivo was studied by using Txbm mice (Thymectomized, irradiated, and stem cell-reconstituted mice). In these mice, TNP-specific CTL could be induced in vitro with TNP-coupled spleen cells only if the cultures were supplemented with an IL 2-containing supernatant (ConAsup). In contrast to normal mice, TNP-specific Lyt-2-TH cells could not be induced by skin painting with trinitrochlorobenzene (TNCB) (as tested by the ability to help CTL formation from thymocyte or normal spleen precursors). These data confirm previous findings that Txbm mice possess CTLp but that their TH compartment is deficient. TNCB skin painting had, however, a clear priming effect on the CTLp population: spleen cells from TNCB-painted mice could give rise to specific CTL with a lower amount of ConAsup than spleen cells from unprimed mice. In addition to this, priming changed the CTLp so that stimulation with lightly coupled cells (0.1 mM trinitrobenzene sulfonic acid (TNBS) instead of 10 mM TNBS) became effective. These changes took place without a significant increase in the frequency of TNP-specific CTL precursors. The data obtained are consistent with the concept that at least with some antigens, CTLp proliferation (clonal expansion), which is probably caused by activated TH cells, is not required for the induction of immunologic memory in vivo.
Research Organization:
Univ. of Helsinki, Finland
OSTI ID:
5005742
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 6; ISSN JOIMA
Country of Publication:
United States
Language:
English

Similar Records

Genetic control of cell-mediated lympholysis to trinitrophenyl (TNP)-modified murine syngeneic cells. Expression of Ir gene function at the cytotoxic precursor and helper cell
Journal Article · Sat Feb 28 23:00:00 EST 1981 · J. Immunol.; (United States) · OSTI ID:5677519
Control of hapten specific contact sensitivity and cytotoxic T cells by orally administered hapten
Journal Article · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6962201
Helper T cells against tumor-associated antigens (TAA): preferential induction of helper T cell activities involved in anti-TAA cytotoxic T lymphocyte and antibody responses
Journal Article · Mon Mar 31 23:00:00 EST 1986 · J. Immunol.; (United States) · OSTI ID:6776780

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL DIFFERENTIATION
CHIMERAS
CHLORINATED AROMATIC HYDROCARBONS
CONNECTIVE TISSUE CELLS
HALOGENATED AROMATIC HYDROCARBONS
IMMUNOLOGY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MEDICINE
MICE
MOSAICISM
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
RADIATION CHIMERAS
RODENTS
SKIN
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
STIMULATION
SURGERY
THYMECTOMY
THYMUS CELLS
VERTEBRATES