Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy dispersive x-ray fluorescence. 1: Technique and determination of the normal reference interval

Journal Article · Mon Apr 01 04:00:00 EST 1985 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5411063

Maier, E A; Rastegar, F; Heimburger, R; Ruch, C; Pelletier, A; Maier, A; Leroy, M J

The authors have applied energy dispersive x-ray fluorescence to the analysis of broncho-alveolar lavage fluids, to determine the concentration of several elements simultaneously with manipulation of the sample minimized. A 20-microL sample of a homogenized lavage fluid, to which two internal standards (containing Y and V) have been added, is deposited on a polypropylene film, 4 microns in thickness, and analyzed by x-ray fluorescence. The authors report the analytical procedure, the detection limits, and the reproducibility. The normal concentration range for trace elements is reported for a selected population. The simplicity and rapidity of the method, and the possibility of automating measurements, make this procedure suitable for screening large numbers of lavage specimens and to establish the diagnosis of some pneumoconioses.

• Research Organization: Ecole Nationale Superieure de Chimie, Strasbourg, France
• OSTI ID: 5411063
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 31:4; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHEMICAL ANALYSIS
EVALUATION
LAVAGE
LUNGS
MATERIALS
NONDESTRUCTIVE ANALYSIS
ORGANS
RESPIRATORY SYSTEM
SCREENING
TRACE AMOUNTS
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS