Simultaneous determination of trace elements in serum by energy-dispersive x-ray fluorescence spectrometry

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6303560

Rastegar, F; Maier, E A; Heimburger, R; Christophe, C; Ruch, C; Leroy, M J

Energy-dispersive x-ray fluorescence is applied in the analysis of human serum to determine the concentrations of several elements simultaneously with minimal manipulation of the sample. The analytical procedure has been developed with standard sera, and standardization, detection limits, and reproducibility have been established. A 50-microL sample of diluted serum, to which an internal standard has been added, is deposited on a thin (4-microns thick) polypropylene film and analyzed by x-ray fluorescence. A report is given of the statistical distributions of the concentrations of Fe, Cu, Zn, and Br obtained in the population (103 samples) studied, and report detection limits for the other 22 elements studied. The simplicity of the method, the high throughput, and the possibility of automating the measurements make this procedure suitable for screening large numbers of sera.

• Research Organization: Nationale Superieure de Chimie, Minerale, Strasbourg, France
• OSTI ID: 6303560
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 30:8; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BLOOD CHEMISTRY
BLOOD SERUM
BROMINE
CHEMICAL ANALYSIS
COPPER
DATA
ELEMENTS
HALOGENS
INFORMATION
IRON
MAMMALS
MAN
METALS
MULTI-ELEMENT ANALYSIS
NONDESTRUCTIVE ANALYSIS
NONMETALS
NUMERICAL DATA
PRIMATES
STATISTICAL DATA
TRANSITION ELEMENTS
VERTEBRATES
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS
ZINC