Cellular bioassay of fish as a sensitive index of water quality related to ash from coal combustion. Technical report

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5410044

Hinton, D E; Walker, E R; Kontir, D M

Static exposure of aqueous suspensions of ash from coal combustion to Oryzias latipes (embryo), Pimephales promelas, and Salmo gairdoeri (adults) was done to determine whether cells of gill, gut, and/or lateral-line system could phagocytose particulates. Energy dispersive X-ray microanalysis of specific cells in gill did not reveal presence of particulates after exposure. Studies on intestine and lateral line are continuing. Effects of uptake of one coal associated polynuclear aromatic, 3-methylcholanthrene (3-MC), were studied in trout liver by a coupled structure/function approach. 3-MC caused induction of liver microsomal benzo(a)pyrene (BP) hydroxylasse activity but did not affect epoxide hydrase activity. In-vitro microsomal metabolism of BP was qualitatively and quantitatively altered by prior 3-MC exposure. No morphologic alterations accompanied biochemical changes. Field studies to assess effects of coal combustion byproducts on feral fish tissues and enzymes are continuing.

• Research Organization: West Virginia Univ., Morgantown (USA). Water Research Inst.
• OSTI ID: 5410044
• Report Number(s): PB-84-110824
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010900* -- Coal
Lignite
& Peat-- Environmental Aspects
3-METHYLCHOLANTHRENE
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOL WASTES
AIR POLLUTION
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ASHES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
CARBONACEOUS MATERIALS
CHEMICAL ANALYSIS
COAL
COMBUSTION PRODUCTS
DISSOLUTION
ENERGY SOURCES
ENVIRONMENTAL QUALITY
FISHES
FLY ASH
FOSSIL FUELS
FUELS
HYDROCARBONS
INDUSTRIAL WASTES
LEACHING
MATERIALS
METABOLISM
ORGANIC COMPOUNDS
PHAGOCYTOSIS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
PONDS
RESIDUES
SEPARATION PROCESSES
SURFACE WATERS
TOXICITY
UPTAKE
VERTEBRATES
WASTES
WATER POLLUTION
WATER QUALITY