Metabolism of 3-methylcholanthrene by rat liver microsomes: a reinvestigation

Journal Article · Fri Dec 31 23:00:00 EST 1976 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)90180-2· OSTI ID:7057792

Stoming, T A ^[1]; Bornstein, W; Bresnick, E

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1. Medical Coll., Augusta, GA

Metabolites of 3-methylcholanthrene (3-MC) formed by rat liver microsomes were analyzed by high pressure liquid chromatography. The metabolic profile is significantly different from previous studies using thin layer chromatography. The major metabolites include 1- and 2-hydroxy-3-MC. Use of the high pressure liquid chromatographic system allows for the separation of at least seven new metabolites. The amounts of three of these new metabolites are substantially decreased when the potent epoxide hydrase inhibitor 3,3,3-trichloropropene oxide is added to the incubation system. These results then suggest the formation of epoxides of 3-methylcholanthrene other than the K-region oxide.

• OSTI ID: 7057792
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 79:2; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AROMATICS
BODY
CARCINOGENESIS
CARCINOGENS
CELL CONSTITUENTS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISEASES
EPOXIDES
GLANDS
HYDROCARBONS
LIVER
MAMMALS
METABOLISM
METABOLITES
MICROSOMES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RODENTS
SEPARATION PROCESSES
VERTEBRATES