Fractionation of an oil-shale retort process water: isolation of photoactive genotoxic components

Conference · Fri Jan 01 04:00:00 EST 1982

OSTI ID:5409366

Strniste, G F; Bingham, J M; Spall, W D; Nickols, J W; Okinaka, R T; Chen, D J.C.

In this report recent data concerning attempts to fractionate and to chemically identify photoactive, genotoxic components in a particularly bioactive oil shale retort process water are presented. Liquid-liquid extraction methods and reverse-phase high pressure liquid chromatography (HPLC) were employed in the fractionation scheme and individual samples were assessed for potential photo-induced toxicity and mutagenicity in cultured Chinese hamster ovary (CHO) cells. For comparison, these fractionated water samples were also assessed for mutagenic potential using the Salmonella histidine reversion assay with microsomal activation (Ames et al., 1975). The photoactive fractions of the process water have been analyzed by gas chromatography/mass spectrometry (GC/MS) to define their chemical composition. Several classes of chemical compounds, including alkylated aromatic hydrocarbons, aromatic amines, aldehydes, amides and ketones are prevalent and most likely contribute to the photo-induced genotoxic potential of this oil shale retort process water.

• Research Organization: Los Alamos National Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 5409366
• Report Number(s): LA-UR-82-1272; CONF-820122-2; ON: DE82010428
• Country of Publication: United States
• Language: English

Related Subjects

04 OIL SHALES AND TAR SANDS
040700* -- Oil Shales & Tar Sands-- Health & Safety
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMIDES
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BITUMINOUS MATERIALS
CARBONACEOUS MATERIALS
CELL CULTURES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
EXPERIMENTAL DATA
EXTRACTION
FOSSIL FUELS
FRACTIONATION
FUELS
FURANS
FURFURAL
GAS CHROMATOGRAPHY
GENETIC EFFECTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INFORMATION
KETONES
LIQUID COLUMN CHROMATOGRAPHY
LIQUID WASTES
MAMMALS
MASS SPECTROSCOPY
MATERIALS
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
NUMERICAL DATA
OIL SHALES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PYRIDINE
PYRIDINES
RADIATIONS
RETORTING
REVERTANTS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
SYNERGISM
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES
WASTE WATER
WASTES
WATER