The absorption, distribution, excretion, and metabolism of a single oral dose of O-ethyl O-4-nitrophenyl phenylphosphonothioate in hens

Journal Article · Mon Aug 01 04:00:00 EDT 1983 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(83)90175-8· OSTI ID:5394749

Abou-Donia, M B; Reichert, B L; Ashry, M A

The disposition and metabolism of a single oral 10 mg/kg (LD50) of uniformly phenyl-labeled (/sup 14/C)EPN (O-ethyl O-4-nitrophenyl (/sup 14/C)phenylphosphonothioate) were studied in adult hens. The birds were protected from acute toxicity with atropine sulfate. Three treated hens were killed at each time interval (days): 0.5, 2, 4, 8, 12. Radioactivity was adsorbed from the gastrointestinal tract and distributed in all tissues. Most of the dose was excreted in the combined urinary-fecal excreta (74%). Only traces of the radioactivity (0.2%) were detected in expired CO/sub 2/. Most of the excreted radioactive materials were identified as phenylphosphonic acid (PPA), O-ethyl phenylphosphonic acid (EPPA), and O-ethyl phenylphosphonothioc acid (EPPTA). Radioactivity in tissues reached a peak of 11.8% in 12 days. The highest concentration of radioactivity was present in the liver followed by bile, kidney, adipose tissue, and muscle. EPN was the major compound identified in brain, spinal cord, sciatic nerve, kidney, and plasma. Most of the radioactivity in the liver was identified as EPPA followed by EPPTA and PPA. Kinetic studies showed that EPN disappeared exponentially from tissues. The half-life of the elimination of EPN from plasma was 16.5 days corresponding to a constant rate value of 0.04 day-1. Relative residence (RR) of EPN relative to plasma was shortest in liver and longest in adipose tissue followed by sciatic nerve and spinal cord.

• Research Organization: Department of Pharmacology, Duke University Medical Center, Durham, North Carolina
• OSTI ID: 5394749
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 70:1; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ADIPOSE TISSUE
ALKALOIDS
ANIMAL TISSUES
ANIMALS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BILE
BIOLOGICAL MATERIALS
BIRDS
BODY
BODY FLUIDS
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CHICKENS
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
FOWL
GLANDS
INSECTICIDES
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LIVER
MATERIALS
METABOLISM
MUSCLES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PESTICIDES
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES