Protein composition and synthesis in the adult mouse spinal cord

Journal Article · Sun May 01 04:00:00 EDT 1983 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00964700· OSTI ID:5589927

Stodieck, L S; Luttges, M W

Properties of spinal cord proteins were studied in adult mice subjected to unilateral crush or electrical stimulation of sciatic nerve. The protein composition of spinal tissue was determined using SDS-polyacrylamide gel electrophoresis coupled with subcellular fractionation. Comparisons of mouse spinal cord and brain revealed similarities in the types but differences in the concentrations of myelin associated proteins, nuclear histones and other proteins. Comparisons with sciatic nerve proteins demonstrated differences in types of proteins but similarities in the concentration of myelin proteins and nuclear histones. The short term (less than 2 hrs.) incorporation of radioactive amino acids into spinal cord proteins revealed heterogeneous rates of incorporation. Neither nerve crush six days prior to testing nor sciatic nerve stimulation had a significant effect on the protein composition or amino acid incorporation rates of spinal cord tissue. These observations suggest that known differences in spinal cord function following alterations in nerve input may be dependent upon different mechanisms than have been found in the brain.

• Research Organization: Department of Aerospace Engineering Sciences, University of Colorado, Boulder
• OSTI ID: 5589927
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 8:5; ISSN NERED
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
ELECTROPHORESIS
INJURIES
ISOTOPES
LIPIDS
LIPOPROTEINS
MAMMALS
METABOLISM
MICE
MYELIN
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECOVERY
REPAIR
RODENTS
SPINAL CORD
SULFUR ISOTOPES
VERTEBRATES