Enhancement of metastatic potential by gamma-interferon

Journal Article · Thu Aug 01 04:00:00 EDT 1991 · Cancer Research; (United States)

OSTI ID:5390861

Kelly, S A; Gschmeissner, S; East, N; Balkwill, F R ^[1]

---

1. Biological Therapy Laboratory, Imperial Cancer Research Fund, London (England)

Preincubation of murine colon 26 colon adenocarcinoma cells with {gamma}-interferon (IFN-gamma), but not {alpha}-interferon, produced a significant increase in experimental pulmonary metastases in syngeneic BALB/c and T-cell-deficient BALB/c nude mice. The enhancement was seen after as little as 1 h of exposure to 1 unit/ml of IFN-{gamma} and persisted for at least 72 h following removal of the cytokine. IFN-{gamma} exerted its effects by increasing the pulmonary retention of cells during the first 6 h following tumor cell injection. During this period all cells visualized in the lung were trapped in pulmonary capillaries. The enhancement was not due to modulations in class I major histocompatibility complex surface antigen expression; nor was it due to alterations in cell size, adhesion to components of the extracellular matrix in vitro, heterotypic or homotypic adhesion, sensitivity to lysis by activated peritoneal macrophages, osmotic fragility, enhancement of surface class II major histocompatibility complex antigen expression, or enhancement of intercellular adhesion molecule-1 (ICAM-1). Colon 26 was completely resistant to natural killer cell-mediated lysis in vitro, and IFN-{gamma} did not modulate the ability of colon 26 to form conjugates with isolated splenocytes. In vivo elimination of anti-asialo GM1 + cells increased pulmonary metastasis, and in such mice, there was no longer a difference in metastatic potential between control and IFN-gamma-treated cells. The authors conclude that low doses of IFN-{gamma} generated at the site of the tumor by host-infiltrating cells or during cytokine therapy could enhance the survival of tumor cells in the circulation and enhance their metastatic potential.

• OSTI ID: 5390861
• Journal Information: Cancer Research; (United States), Journal Name: Cancer Research; (United States) Vol. 51:15; ISSN 0008-5472; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BODY
CARCINOMAS
CELL DIVISION
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
EXPERIMENTAL NEOPLASMS
GASTROINTESTINAL TRACT
GENES
GROWTH FACTORS
INTERFERON
INTESTINES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LARGE INTESTINE
LUNGS
LYMPHOKINES
MACROPHAGES
MAMMALS
METASTASES
MICE
MITOGENS
NEOPLASMS
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES