Mutational specificities of environmental carcinogens in the lacl gene of Escherichia coli H. V: DNA sequence analysis of mutations in bacteria recovered from the liver of Swiss mice exposed to 1,2-dimethylhydrazine, azoxymethane, and methylazoxymethanolacetate

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Mol. Carcinog; (United States)

DOI:https://doi.org/10.1002/mc.2940040304· OSTI ID:5388843

Zeilmaker, M J; Horsfall, M J; van Helten, J B; Glickman, B W; Mohn, G R ^[1]

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1. Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, University of Leiden (Netherlands)

The host-mediated assay (HMA) was used to determine the spectra of mutations induced in the lacl gene of Escherichia coli cells recovered from the livers of Swiss mice exposed to the carcinogens 1,2-dimethylhydrazine (SDMH), azoxymethane (AOM), and methylazoxymethanolacetate (MAMA). These spectra were further compared with changes induced by dimethylnitrosamine (DMNA) in the HMA methodology. A total of 177 independent lacl mutations arising in the HMA following exposure to SDMH, AOM, and MAMA were analyzed. Single-base substitutions accounted for 97% of all mutations analyzed. The vast majority of the single-base substitutions consisted of G:C----A:T transitions (94% of all mutations). The remaining mutations consisted of A:T----G:C transitions (3% of all mutations) while non-base substitutions accounted for only 3% of the total mutagenesis. The latter mutations consisted of one frameshift mutation and four lacO deletions. The distribution of G:C----A:T transitions induced by the three chemicals in the first 200 bp of the lacl gene was not random, but rather clustered at sites where a target guanine was flanked at the 5{prime} site by a purine residue.

• OSTI ID: 5388843
• Journal Information: Mol. Carcinog; (United States), Journal Name: Mol. Carcinog; (United States) Vol. 4:3; ISSN XY971; ISSN 0899-1987
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMALS
BACTERIA
BODY
CARCINOGENS
DIGESTIVE SYSTEM
DNA SEQUENCING
ESCHERICHIA COLI
GENE MUTATIONS
GLANDS
LIVER
MAMMALS
MICE
MICROORGANISMS
MUTAGENESIS
MUTATIONS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES