Changes in DNA base sequence induced by gamma-ray mutagenesis of lambda phage and prophage
Journal Article
·
· Genetics; (United States)
OSTI ID:7195239
Mutations in the cI (repressor) gene were induced by gamma-ray irradiation of lambda phage and of prophage, and 121 mutations were sequenced. Two-thirds of the mutations in irradiated phage assayed in recA host cells (no induction of the SOS response) were G:C to A:T transitions; it is hypothesized that these may arise during DNA replication from adenine mispairing with a cytosine product deaminated by irradiation. For irradiated phage assayed in host cells in which the SOS response had been induced, 85% of the mutations were base substitutions, and in 40 of the 41 base changes, a preexisting base pair had been replaced by an A:T pair; these might come from damaged bases acting as AP (apurinic or apyrimidinic) sites. The remaining mutations were 1 and 2 base deletions. In irradiated prophage, base change mutations involved the substitution of both A:T and of G:C pairs for the preexisting pairs; the substitution of G:C pairs shows that some base substitution mechanism acts on the cell genome but not on the phage. In the irradiated prophage, frameshifts and a significant number of gross rearrangements were also found.
- Research Organization:
- Yale Univ., New Haven, CT (USA)
- OSTI ID:
- 7195239
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 118:4; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA MISMATCH
DNA REPAIR
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
MUTATIONS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA MISMATCH
DNA REPAIR
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
MUTATIONS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES