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Mutational specificities of environmental carcinogens in the lacl gene of Escherichia coli. III. The cyclic nitrosamine N-nitrosopyrrolidine is a complex mutagen

Journal Article · · Mol. Carcinog; (USA)
; ;  [1]
  1. York Univ., Toronto, Ontario (Canada)
+ Show Author Affiliations
The mutational specificity of the cyclic nitrosamine N-nitrosopyrrolidine (NPYR) was determined through the DNA sequence characterization of 33 lacl-d mutations of Escherichia coli. Base substitution was the predominant class of mutation induced (91%). The majority of these (64%) occurred at GC base pairs, in accordance with the predicted significance of NPYR-derived guanine adducts. In addition, this nitrosamine efficiently produced other kinds of base substitution events as 11 of the 33 mutations occurred at AT base pairs. Deletion, frameshift, and duplication events were also recovered. The complexity of the NPYR mutational spectrum appears to be consistent with the suggestion that this compound acts through both direct and indirect mutational pathways.
OSTI ID:
6518518
Journal Information:
Mol. Carcinog; (USA), Journal Name: Mol. Carcinog; (USA) Vol. 3:3; ISSN 0899-1987; ISSN XY971
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AMINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
DNA ADDUCTS
DNA SEQUENCING
ESCHERICHIA COLI
GENE MUTATIONS
GENES
GENETIC EFFECTS
MICROORGANISMS
MUTAGENESIS
MUTATIONS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SENSITIVITY
STRUCTURAL CHEMICAL ANALYSIS