Lung sequestration in vivo of activated, tritium-labeled rabbit neutrophils

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5386268

Lafuze, J; Baker, M; Oakes, A; Landes, C; Gasta, T P

Lung endothelium may be damaged as it interacts with blood neutrophils (PMN) activated by certain bacterial filtrates. N-formyl-methionyl-leucyl-phenylalanine (FMLP) synthetic analog of E. coli endotoxin, causes transient neutropenia and respiratory distress when infused i.v. in rabbits. In this study the authors determined lung, spleen, liver and kidney sequestration of activated PMN. The authors transfused blood from a donor pre-treated with 3 mCi of (/sup 3/H)-thymidine into test and control (CON) rabbits. PMN were activated with 0.2 ug/Kg of FMLP i.v. Lungs, spleen, liver and kidneys were removed at 2.5 min. and were homogenized. Counts of 100 mg (wet weight) of each organ minus dpm of 100 mg CON were compared. Results were: lung 1242, spleen 448, kidney 235 and liver 209 (n = 10). Values for lung were significantly different from spleen (p < .03), kidney (p < .01) and liver (p < .01). Using the same model, the authors infused FMLP or vehicle and withdrew 6 ml venous blood at intervals. Test and CON values were compared. Each was calculated as percent change from time zero. Results were: at 2.5 min (+ FMLP) - 95% vs (CON) - 1% (p < .0001), at 20 min -69 vs -24 (p < .01), at 40 min -52 vs -19 (p < .01), and at 60 min -27 vs -25 (n.s.). These results indicate that on a per unit wt basis the lung sequestration of FMLP-activated PMN in vivo is significantly greater than that of spleen, kidney or liver and that the same cells sequestered are returned to the peripheral circulating pool.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis
• OSTI ID: 5386268
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOTHELIUM
GLANDS
HETEROCYCLIC COMPOUNDS
IN VIVO
INJURIES
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
LIVER
LUNGS
MAMMALS
MATERIALS
NEUTROPHILS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHENYLALANINE
PYRIMIDINES
RABBITS
RESPIRATION
RESPIRATORY SYSTEM
RIBOSIDES
SPLEEN
THERAPY
THYMIDINE
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
TRANSFUSIONS
TRITIUM COMPOUNDS
VERTEBRATES