A new method to evaluate extravascular albumin and blood cell accumulation in the lung

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Applied Physiology (1985); (USA)

OSTI ID:5227989

Bureau, M F; Malanchere, E; Pretolani, M; Boukili, M A; Vargaftig, B B ^[1]

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1. Unite Associee Institut Pasteur-Institut National de la Sante et de la Recherche Medicale, Paris (France)

A method was developed to evaluate blood volume, accumulation of extravascular albumin (ALBev), and platelet (PL) or polymorphonuclear neutrophil (PMN) sequestration in lungs after challenge with inflammatory agents. Erythrocytes (RBC), albumin, and PL or PMN, labeled with 99mTc, 131I, and 111In,-respectively, were injected intravenously into anesthetized and ventilated guinea pigs. The different parameters were calculated from in vivo lung and blood radioactivity values. When N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) was injected intravenously at 10 micrograms.kg-1, lung RBC content dropped by 14.7 +/- 1.8% (SE; n = 10), indicating a reduced lung blood volume, ALBev rose to 15.0 +/- 3.2% of the initial albumin vascular content, and the circulating PMN were sequestered by 9.2 +/- 1.7%. A transient PL sequestration was also observed 1 min after the injection of fMLP (13.1 +/- 2.0%, n = 7). During the infusion of 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine, the lung PL content rose dose dependently from 10.1 +/- 2.2% of the circulating pool with 3 ng.kg-1.min-1 to 54.9 +/- 20.1% with 44 ng.kg-1.min-1, the lung RBC content decreased by greater than 10%, and the ALBev increased beyond 16%. Our method allows the study of the correlations between cell entrapment and the variations of the albumin exchanges in the lung and may lead to a better understanding of the correlations between cell activation and edema.

• OSTI ID: 5227989
• Journal Information: Journal of Applied Physiology (1985); (USA), Journal Name: Journal of Applied Physiology (1985); (USA) Vol. 67:4; ISSN 8750-7587; ISSN JAPHE
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALBUMINS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD PLATELETS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EDEMA
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
ESTERS
EXTRACELLULAR SPACE
GUINEA PIGS
HOURS LIVING RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IODINE 131
IODINE ISOTOPES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LUNGS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEUROREGULATORS
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PATHOLOGICAL CHANGES
PNEUMONIA
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SPACE
SYMPTOMS
TECHNETIUM 99
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES