Neutrophhil function after exposure to polychlorinated biphenyls in vitro

Journal Article · Fri Oct 01 00:00:00 EDT 1993 · Environmental Health Perspectives; (United States)

DOI:https://doi.org/10.1289/ehp.93101430· OSTI ID:5483066

Ganey, P E; Denison, M; Roth, R A; Robinson, J P ^[1]

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1. Michigan State Univ., East Lansing, MI (United States)

Polychlorinated biphenyls (PCBs) are known to be immunotoxic, yet the effects on neutrophil (PMN) function are not well characterized. We incubated PMNs isolated from rat peritoneum with a mixture of PCB congeners, Aroclor 1242, in the absence or presence of either phorbol myristate acetate (PMA) to stimulate generation of supoxide anion (O[sub 2]) or N-formyl-methionyl-leucyl-phenylalanine (fMLP) to induce degranulation (measured as release of [beta]-glucuronidase). Aroclor 1242 alone stimulated O[sub 2] production at a concentration of 10 [mu]g/ml. Significant cytotoxicity was not observed under these conditions. This concentration of Aroclor 1242 also increased O[sub 2] generation in PMNs activated with 20 ng PMA/ml. In the presence of a concentration of PMA (2 ng/ml) that by itself did not stimulate production of O[sub 2], 1 [mu]g Aroclor 1242/ml caused significant generation of O[sub 2], indicating synergy between Aroclor 1242 and PMA. Aroclor 1242 caused release of [beta]-glucuronidase from quiescent PMNs; however, in PMNs stimulated with fMLP to undergo degranulation, Aroclor 1242 inhibited release of [beta]-glucuronidase.

• OSTI ID: 5483066
• Journal Information: Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 101:5; ISSN 0091-6765; ISSN EVHPAZ
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHLORINATED AROMATIC HYDROCARBONS
DOSE-RESPONSE RELATIONSHIPS
DYNAMIC FUNCTION STUDIES
HALOGENATED AROMATIC HYDROCARBONS
KINETICS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
NEUTROPHILS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
POLYCHLORINATED BIPHENYLS
PROTEINS
REACTION KINETICS
RECEPTORS
TOXICITY