Further studies on the late preventive effects of the anticalmodulin trifluoperazine on carbon tetrachloride-induced liver necrosis

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5382087

Castro, J; Bernacchi, A; Fernandez, G; Villarruel, M C; Ferreyra, E; Castro, C; Fenos, O

The authors previously reported that trifluorperazine (TFP) administration 6 or 10 hr after CCl/sub 4/ is able to partially prevent liver necrosis by the hepatotoxin at 24 hr. Preventive effect is not due to interference by TFP on CCl/sub 4/ metabolic activation to CCl/sub 3/ or its covalent binding to cellular components or of lipid peroxidation. Now the authors report that TFP administration 10 hr after CCl/sub 4/ does not prevent calcium accumulation but increases glycogen content. Increases in glycogen are more marked in livers of animals receiving only TFP. Administration slightly stimulates /sup 14/C-leucine incorporation in liver proteins but it does not modify decay of radioactivity in (/sup 14/C-guanidino) arginine prelabelled liver protein. TFP does not modify decay of radioactivity in /sup 32/P prelabelled phospholipid. Electron microscopy studies of livers from CCl/sub 4/ poisoned rats receiving TFP 10 hr after the hepatoto toxin and sacrificed at 24 hr revealed the presence of glycogen granules in otherwise glycogen-depleted preparations. These preparations showed only slight dilatation of the endoplasmic reticulum or the perinuclear membrane and intact mitochondria. Results might suggest that TFP interaction with calmodulin might interfere with a process of propagation of CCl/sub 4/-induced liver damage sparked by calcium accumulation and requiring the hormone for operation.

• Research Organization: CEITOX, Villa Martelli, Argentina
• OSTI ID: 5382087
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
ANTIDEPRESSANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON TETRACHLORIDE
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM AGENTS
CHLORINATED ALIPHATIC HYDROCARBONS
COVALENCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
ESTERS
GLANDS
GLYCOGEN
HALOGENATED ALIPHATIC HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
LIGHT NUCLEI
LIPIDS
LIVER
MAMMALS
MEMBRANES
METABOLIC ACTIVATION
MICROSCOPY
MITOCHONDRIA
NECROSIS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PATHOLOGICAL CHANGES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
PSYCHOTROPIC DRUGS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES