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Effect of chlordecone on carbon tetrachloride-induced increase in calcium uptake in isolated perfused rat liver

Journal Article · · Toxicol. Appl. Pharmacol.; (United States)
;
Intracellular accumulation of Ca/sup 2 +/ can occur in the livers of animals poisoned with a toxic dose of CCl/sub 4/. We have reported even greater accumulation of cytosolic Ca/sup 2 +/ in animals treated with an ordinarily nontoxic dose of CCl/sub 4/ in combination with prior exposure to chlordecone (CD). Present studies were designed to examine if intact perfused livers obtained from animals receiving either CCl/sub 4/ (100 microliter/kg, ip) alone or in combination with prior dietary exposure to 10 ppm CD for 15 days accumulated /sup 45/Ca from the perfusate. Livers obtained at 0, 1, 4, 6, 12, 24, and 36 hr after a single CCl/sub 4/ injection were perfused with Krebs-Ringer bicarbonate buffer containing erythrocytes, bovine serum albumin, and dextrose. After a 15-min equilibration, /sup 45/Ca was added to the perfusate, and the perfusion was continued for 30 min. Hepatic /sup 45/Ca accumulation in CCl/sub 4/-treated animals in the whole liver or in the subcellular organelles obtained from perfused liver was not significantly different from corn oil controls. In the CD + CCl/sub 4/ combination treatment, /sup 45/Ca accumulation followed a biphasic pattern with the first increase at 1 hr and then a progressive rise starting at 12 hr after CCl/sub 4/ administration. Mitochondrial, microsomal, and cytosolic fractions from perfused liver showed a progressive rise in /sup 45/Ca at late time periods, which is indicative of unregulated and irreversible influx of extracellular Ca/sup 2 +/ into these livers. These studies demonstrate that cytosolic Ca/sup 2 +/ progressively increases as a result of the unregulated entry of extracellular Ca/sup 2 +/.
Research Organization:
Univ. of Mississippi Medical Center, Jackson
OSTI ID:
5527725
Journal Information:
Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARBON TETRACHLORIDE
CHLORINATED ALIPHATIC HYDROCARBONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
EVEN-ODD NUCLEI
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
INSECTICIDES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KEPONE
LIVER
MAMMALS
METALS
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PERFUSED ORGANS
PESTICIDES
RADIOISOTOPES
RATS
RODENTS
TOXICITY
TRACER TECHNIQUES
UPTAKE
VERTEBRATES