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Role of suppressed hepatocellular regeneration and Ca/sup 2 +/ in chlordecone-potentiated CCl/sub 4/ hepatotoxicity

Thesis/Dissertation ·
OSTI ID:6411340
The mechanism by which the chlorinated pesticide chlordecone (CD; Kepone) potentiates CCl/sub 4/-induced hepatotoxicity and lethality was investigated. It was hypothesized that perturbations in Ca/sup 2 +/ homeostasis, greater than those observed with a low dose of CCl/sub 4/ alone, in concert with a suppression of hepatocellular regeneration induced by CD alone or by CD + CCl/sub 4/ are responsible, at least in part, for CD-potentiated CCl/sub 4/ hepatotoxicity. Ca/sup 2 +/ homeostasis was evaluated by measuring total cell Ca/sup 2 +/ and /sup 45/Ca/sup 2 +/ uptake in viable isolated hepatocyte suspension obtained from normal and CD-pretreated rats receiving CCl/sub 4/ in vivo. In the normal rats in vivo CCL challenge did not affect /sup 45/Ca/sup 2 +/ uptake by viable isolated hepatocytes. In contrast, /sup 45/Ca/sup 2 +/ uptake was inhibited in viable isolated hepatocytes obtained from rats exposed to CD + CCl/sub 4/.
Research Organization:
Mississippi Univ., Jackson (USA). Medical Center
OSTI ID:
6411340
Country of Publication:
United States
Language:
English

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