Effect of alpha-naphthyl isothiocyanate and CCl/sub 4/ interaction on hepatocellular calcium transport

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Bull. Environ. Contam. Toxicol.; (United States)

DOI:https://doi.org/10.1007/BF01699977· OSTI ID:5813701

Agarwal, A K; Zinermon, W D

Alpha-naphthyl isothiocyanate (ANIT), and CCl/sub 4/ are both hepatotoxic agents but with different mechanisms of action. In recent years, much attention has been focused on the disruption of intracellular calcium homeostasis as a common ultimate mechanism of the chemical induced irreversible hepatocellular damage. Calcium is known to accumulate in necrotic tissues. The extracellular fluid is rich in calcium whereas the concentration of the intracellular calcium is much lower. Therefore, it is believed that any damage to plasma membrane will result in an influx of calcium. Calcium ions are biologically very active, being capable of considerable disruption of metabolic order. Agents such as CCl/sub 4/, BrCCl/sub 3/ which cause hepatocellular necrosis have also been reported to perturb the intracellular calcium homeostasis. The present study was designed to determine the effect of intrahepatic cholestasis as produced by a single toxic dose of ANIT on CCl/sub 4/ induced hepatotoxicity and hepatocellular calcium uptake. /sup 45/Ca uptake in the isolated mitochondria and microsomes using three different doses of CCl/sub 4/ known to be toxic was measured in ANIT pretreated animals. Calcium ATPase in these organelles was assayed. Microsomal cytochrome P-450 and the levels of serum GPT and Bilirubin were also determined.

• Research Organization: City Univ. of New York, NY (USA)
• OSTI ID: 5813701
• Journal Information: Bull. Environ. Contam. Toxicol.; (United States), Journal Name: Bull. Environ. Contam. Toxicol.; (United States) Vol. 42:3; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560300* -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBON TETRACHLORIDE
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHLORINATED ALIPHATIC HYDROCARBONS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
INTERMEDIATE MASS NUCLEI
IONS
ISOTHIOCYANATES
ISOTOPES
LIVER
LIVER CELLS
MAMMALS
MEMBRANE TRANSPORT
MICROSOMES
MITOCHONDRIA
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
RADIOISOTOPES
RATS
RIBOSOMES
RODENTS
SCINTILLATION COUNTING
SOMATIC CELLS
SYNERGISM
VERTEBRATES