1,25 (OH) sub 2 vitamin D sub 3 -induced sup 45 Ca uptake in vascular myocytes cultured from spontaneously hypertensive and normotensive rats

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90111-N· OSTI ID:5381664

Xue, Hong; McCarron, D A; Bukoski, R D ^[1]

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1. Oregon Health Sciences Univ., Portland (USA)

The effect of 1,25 (OH){sub 2} vitamin D{sub 3} on basal {sup 45}Ca uptake was examined in vasvular smooth muscle cells cultured from mesenteric arteries of spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) normotensive rats. Basal uptake of {sup 45}Ca was significantly greater in myocytes of WKY than SHR at 5, 10, 30 and 60 min incubation with the isotope. Incubation with 1 ng/ml 1,25 (OH){sub 2} vitamin D{sub 3} for 48 hr increased basal {sup 45}Ca uptake between 1-10 min in SHR and between 5-10 min in WKY. The dose-response relationship indicated that cells from both strains are equally sensitive to the calciotropic effects of 1,25 (OH){sub 2} vitamin D{sub 3} with half-maximal stimulation occurring at approximately 0.3-0.4 ng/ml. In cells of both strains maximal stimulation of {sup 45}Ca uptake was achieved only after a 12-24 hr period of incubation with hormone and pretreatment with cycloheximide inhibited 1,24 (OH){sub 2} vitamin D{sub 3}-enhanced {sup 45}Ca uptake. Although {sup 45}Ca binding by extracellular matrix material was significantly greater in WKY than SHR, 1,25 (OH){sub 2} vitamin D{sub 3} had no effect on the amount of matrix {sup 45}Ca binding in either strain.

• OSTI ID: 5381664
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 49:9; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL VARIABILITY
BLOOD VESSELS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
EVEN-ODD NUCLEI
GENETIC VARIABILITY
HEART
HYPERTENSION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE TRANSPORT
METALS
NUCLEI
ORGANS
PATHOGENESIS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
VITAMIN D
VITAMINS