Rapid changes in skeletal muscle calcium uptake induced in vitro by 1,25-dihydroxyvitamin D/sub 3/ are suppressed by calcium channel blockers

Journal Article · Fri May 01 04:00:00 EDT 1987 · Endocrinology; (United States)

OSTI ID:6477338

de Boland, A R; Boland, R L

Previous investigations have shown that 1,25-dihydroxyvitamin D/sub 3/ (1,25-(OH)/sub 2/D/sub 3/) stimulates muscle Ca uptake through a nuclear mechanism. The possibility that 1,25-(OH)/sub 2/D/sub 3/ would induce rapid changes in muscle Ca fluxes independent of de novo protein synthesis was investigated in the present work. In vitro preparations of soleus muscles obtained from vitamin D-deficient chicks were used. A significant increase in /sup 45/Ca labeling of the tissue was already observed after 3-min treatment with 2.4 X 10(-10) M 1,25-(OH)/sub 2/D/sub 3/. This early stimulation in muscle Ca uptake became maximal at 10-15 min. Cycloheximide (50 microM) did not block the effect of the metabolite at 15 and 30 min. However, the antibiotic effectively blocked the increase in Ca uptake induced by 1,25-(OH)/sub 2/D/sub 3/ after 1-h treatment. The rapid 1,25-(OH)/sub 2/D/sub 3/-dependent stimulation of /sup 45/Ca labeling of soleus muscle was not associated to changes in lipid synthesis as assessed by measurements of /sup 3/H-glycerol incorporation into the tissue lipids. However, the calcium antagonists verapamil and nifedipine (50 microM) abolished the stimulation in Ca uptake produced by 1,25-(OH)/sub 2/D/sub 3/ in 5 min. These results suggest that 1,25-(OH)/sub 2/D/sub 3/ can act directly at the muscle membrane level affecting Ca fluxes through Ca channels.

• Research Organization: Universidad Nacional del Sur, Bahia Blanca, Argentina
• OSTI ID: 6477338
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 5; ISSN ENDOA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIRDS
CALCIUM 45
CALCIUM ISOTOPES
CHEMISTRY
CHICKENS
CHOLECALCIFEROL
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FOWL
FUNGICIDES
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
LIPIDS
MUSCLES
NUCLEI
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
PESTICIDES
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
SYMPATHOLYTICS
UPTAKE
VERTEBRATES
VITAMIN D
VITAMINS