Pulmonary serotonin and histamine in experimental asbestosis

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5381639

Keith, I M; Day, R; Lemaire, S

Adult male Wistar rats were treated once with tracheal instillation of 5 mg Crysotile B asbestos fibers in 0.5 ml saline under ketamine/xylaxine anesthesia. Control rats (n = 37) received 0.5 ml saline. Test and control rats were killed at 7 and 14 d., and 1, 3 and 6 mo. post instillation. Serotonin (5-HT) was quantitated in lung tissue homogenate from all rats using HPLC and electrochemical detection. Among rats killed at 1, 3 and 6 mo., lung tissue histamine-o-phthaldialdehyde complex was quantitated using reverse phase HPLC coupled to a fluorometric detector. Furthermore, 5-HT was quantitated in the cytoplasm of grouped (NEB) and individual (NEC) neuroendocrine cells and in mast cells using formaldehyde-vapor-induced fluorescence and microspectrofluorometry, and mast cell numbers were determined. Test rats had higher pulmonary 5-HT and histamine levels than controls at 1, 3 and 6 mo. Test rats also had higher cellular 5-HT compared to controls in NEB's at 1 mo., but not in NECs, and tended to have higher 5-HT-levels in mast cells at 6 mo. Mast cell numbers were higher among tests at 1 and 3 mo. The authors results suggest that NEBs may contribute to the early asbestos induced rise in 5-HT, and that the major source of 5-HT and histamine is from the increased numbers of mast cells.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5381639
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550900 -- Pathology
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
ASBESTOS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BODY
CELL CONSTITUENTS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
CYTOPLASM
DISEASES
DRUGS
FLUORESCENCE
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROXY COMPOUNDS
IMIDAZOLES
INDOLES
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
LUNGS
MAMMALS
MAST CELLS
NERVE CELLS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SEPARATION PROCESSES
SEROTONIN
SOMATIC CELLS
SYMPATHOMIMETICS
TRYPTAMINES
VERTEBRATES