The effects of histamine and prostaglandin D2 on rat mast-cell cyclic AMP and mediator release
The possibility that histamine may play a functional role in modulating mast-cell secretion, as has been suggested for basophil degranulation, has both physiologic and pharmacologic implications. Therefore the capacity of histamine to influence rat peritoneal mast-cell (RPMC) cyclic AMP levels and reversed anaphylatic degranulation as reflected in the release of 3H-serotonin (5-HT) was examined. To ascertain that RPMC were functionally responsive to exogenous hormonal stimulation, assessment of prostaglandin (PG) D2 effects on cyclic AMP and 5-HT release were determined in parallel. Although PGD2 (100 microM) increased cyclic AMP and inhibited 5-HT release in the presence of 50 microM aminophylline, histamine (up to 1000 microM) was ineffective was ineffective in both. However, 1000 microM histamine in the presence of 500 microM aminophylline was capable of transiently increasing RPMC cyclic AMP (for 15 to 30 sec) and under these conditions of suppressing 5-HT release. The receptor subtype involved in the suppressive actions of histamine appeared to be of the H-1 type as reflected in the capacity of specific H-1 agonists to reproduce the inhibition of 5-HT release, whereas neither H-2 agonists nor H-2 antagonists had any influence. Thus, under conditions in which phosphodiesterase enzymatic action is impaired, histamine in extremely high concentrations is able to modulate mast-cell secretion. However, it seems very unlikely that this action of histamine has any physiologic significance.
- Research Organization:
- Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD
- OSTI ID:
- 5394440
- Journal Information:
- J. Allergy Clin. Immunol.; (United States), Vol. 68:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dual effects of protoporphyrin and long wave ultraviolet light on histamine release from rat peritoneal and cutaneous mast cells
Release of histamine from mast cells by a synthetic ionophore
Related Subjects
HISTAMINE
BIOCHEMISTRY
MAST CELLS
PHYSIOLOGY
PROSTAGLANDINS
TRITIUM COMPOUNDS
TRACER TECHNIQUES
AMP
RATS
RECEPTORS
SECRETION
SEROTONIN
AMINES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
CHEMISTRY
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMIDAZOLES
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques