Long-term repair in vivo of colony-forming ability and chromosomal injury in X-irradiated mouse hepatocytes
Journal Article
·
· Radiat. Res.; (United States)
The radiosensitivity of mouse hepatocytes in vivo was measured in terms of clonogenicity or chromosome damage (micronucleus production). Within 24 h of irradiation there was a dose-dependent increase in clonogenicity (dose-modifying factor, DMF = 1.37 +/- 0.09) followed by long-term repair which resulted in a DMF of 3.49 +/- 0.23 at 11 months. Such repair also took place, but to a lesser extent, after the end of fractionated irradiation. Cell proliferation, measured by tritiated thymidine autoradiography, was insufficient to explain the long-term reduction in radiosensitivity in terms of a dose-dependent replacement of damaged cells. Although there was a reduction in the frequency of cells with micronuclei, postirradiation, the magnitude of this decrease was relatively small; the DMF for micronucleus-free cells at 11 months was only 1.49 +/- 0.25. Thus, the long-term increase in clonogenicity can only partially be explained in terms of repair of chromosome injury, assessed by the production of micronuclei.
- Research Organization:
- Paterson Institute for Cancer Research, Manchester (England)
- OSTI ID:
- 5379915
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 113:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL NUCLEI
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
COLONY FORMATION
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
HETEROCYCLIC COMPOUNDS
IN VIVO
IONIZING RADIATIONS
IRRADIATION
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
MICE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STEM CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL NUCLEI
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
COLONY FORMATION
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
HETEROCYCLIC COMPOUNDS
IN VIVO
IONIZING RADIATIONS
IRRADIATION
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
MICE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STEM CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION