Frequency of micronuclei in hepatocytes following X and fast-neutron irradiations--an analysis by a linear-quadratic model

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577742· OSTI ID:6271653

Ono, K; Nagata, Y; Akuta, K; Abe, M; Ando, K; Koike, S ^[1]

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1. Kyoto Univ. (Japan)

The usefulness of the micronucleus assay for investigating the radiation response of hepatocytes was examined. The frequency was defined as the ratio of the total number of micronuclei to the number of hepatocytes examined. The dose-response curves were curvilinear after X rays and linear after neutrons. These dose-response curves were analyzed by a linear-quadratic model, frequency = aD + bD2 + c. The a/b ratio was 3.03 +/- 1.26 Gy following X irradiation. This value is within the range of the alpha/beta ratios reported by others using the clonogenic assay of hepatocytes. While the a/b value for neutrons was 24.3 +/- 11.7 Gy, the maximum relative biological effectiveness of neutrons was 6.30 +/- 2.53. Since the micronucleus assay is simple and rapid, it may be a good tool for evaluating the radiation response of hepatocytes in vivo.

• OSTI ID: 6271653
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 123:3; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

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560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
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FAST NEUTRONS
FERMIONS
HADRONS
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NEUTRONS
NUCLEONS
RADIATION EFFECTS
RADIATIONS
RBE
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X RADIATION