IL-1. alpha. increases arachidonyl-CoA: Lysophospholipid acyltransterase activity and stimulates ( sup 3 H) arachidonate incorporation into phospholipids in rat mesangial cells

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(92)90574-9· OSTI ID:5378543

Nakazato, Y; Sedor, J R ^[1]

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1. Case Western Reserve Univ., Cleveland, OH (United States)

The proinflammatory cytokine interleukin-1{alpha} is a potent stimulus of prostaglandin synthesis. The authors have previously shown that IL-1 amplifies mesangial cell prostaglandin synthesis by inducing synthesis of a non-pancreatic phospholipase A{sub 2}. Phospholipase A{sub 2}. Phospholipase A{sub 2} activation results in the formation of lysophospholipids and free fatty acids. They now investigate the effects of IL-1{alpha} on reacylation of lysophospholipids. Incubations with IL-1{alpha} for 24 hours significantly stimulated mesangial cell ({sup 3}H)arachidonic acid incorporation but not ({sup 3}H)oleic acid incorporation into phosphatidylinositol and phosphatidylethanolamine. Lysophospholipid acyltransferase activity was measured in vitro. Cytokine treatment increased enzyme activity when lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylinositol were used as exogenous substrates. They conclude that IL-1 promotes cellular phospholipid remodeling by stimulating the deacylation and reacylation of phospholipids.

• OSTI ID: 5378543
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 50:26; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ANIMALS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ENZYME ACTIVITY
ENZYMES
ESTERS
GROWTH FACTORS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
LYMPHOKINES
MAMMALS
METABOLISM
MITOGENS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PROTEINS
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES