v-myb transformation of Xeroderma pigmentosum human fibroblasts: Overexpression of the c-Ha-ras oncogene in the transformed cells

Journal Article · Tue Oct 01 04:00:00 EDT 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90266-W· OSTI ID:5377066

Michelin, S; Varlet, I; Sarasin, A; Suarez, H G ^[1]; Martinerie, C; Perbal, B ^[2]

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1. Inst. de Recherches Scientifiques sur le Cancer, Villejuif (France)
2. Centre Univ., Orsay (France)

Human Xeroderma pigmentosum normal' fibroblasts AS16 (XP4 VI) were transformed after transfection with a recombinant v-myb clone. In this clone (pKXA 3457) derived from avian myeloblastosis virus (AMV), the expression of the oncogene sequences is driven by the AMV U-5 LTR promoter. The transformed cells (ASKXA), which have integrated a rearranged v-myb oncogene, grow in agar, are not tumorigenic in nude mice, and express a 45-kDa v-myb protein. The HMW DNA of these cells transform chicken embryo fibroblasts. The c-Ha-ras oncogene is overexpressed in the ASKXA cells but not in the parental normal' AS16 cells and a revertant clone (ASKXA Cl 1.1 G). The results lead to the conclusion that the XP fibroblasts are phenotypically transformed by the presence of the transfected v-myb oncogene, which is able to induce an overexpression of the c-Ha-ras gene.

• OSTI ID: 5377066
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 196:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIRDS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
FIBROBLASTS
GENES
MAMMALS
MAN
MICE
ONCOGENES
ONCOGENIC TRANSFORMATIONS
PHENOTYPE
PRIMATES
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
XP CELLS