Both cross-links and monoadducts induced in DNA by psoralens can lead to sister chromatid exchange formation

Journal Article · Sun Sep 01 04:00:00 EDT 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90464-6· OSTI ID:5376827

Cortes, F ^[1]; Morgan, W F; Varcarcel, E R; Cleaver, J E; Wolff, S ^[2]

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1. Univ. of California, San Francisco (United States) Facultad de Biologia, Sevilla (Spain)
2. Univ. of California, San Francisco (United States)

The relative importance of DNA-DNA cross-links and bulky monoadducts in sister chromatid exchange (SCE) formation was investigated in three human fibroblast cell lines with different repair capabilities. These cell lines included normal cells, which can repair both classes of lesion; xeroderma pigmentosum (XP) cells, which cannot repair either psoralen-induced cross-links or monoadducts; and an XP revertant that repairs only cross-links and not monoadducts. SCEs were induced by two psoralen derivatives. After activation with long-wave ultraviolet light, HMT produces cross-links and monoadducts in DNA, whereas 5-MIP produces only monoadducts. In normal human cells both psoralens induced SCEs, but if cells were allowed to repair for 18 h before bromodeoxyuridine (BrdUrd) was added for SCE analysis, the SCE frequency was significantly reduced. XP cells showed an SCE frequency that remained high regardless of whether SCEs were analyzed immediately after psoralen exposure of 18 h later. In the XP revertant that repairs only cross-links, both psoralens induced a high yield of SCEs when BrdUrd was added immediately after psoralen treatment. These observations indicate that both cross-links and monoadducts are lesions in DNA that can lead to SCE formation.

• DOE Contract Number: AC03-76SF01012
• OSTI ID: 5376827
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 196:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL CELLS
ANTICOAGULANTS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CHEMICAL REACTIONS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
COUMARINS
CROSS-LINKING
DNA ADDUCTS
DNA REPAIR
DRUGS
FIBROBLASTS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MOLECULAR BIOLOGY
MUTANTS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
POLYMERIZATION
PSORALEN
PYRIMIDINES
RADIATIONS
REPAIR
REVERTANTS
RIBOSIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
URACILS
XP CELLS