Epidermal growth factor induces tyrosine hydroxylase in a clonal pheochromocytoma cell line, PC-G2

Journal Article · Sat Mar 01 04:00:00 EST 1980 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.84.3.495· OSTI ID:5375152

Goodman, R; Slater, E; Herschman, H R

We have previously described the isolation of a clonal cell line (PC-G2) in which the level of tyrosine hydroxylase (TH), the rate-limiting step in the synthesis of the catecholamine neurotransmitters, is induced by nerve growth factor (NGF). We now report that epidermal growth factor (EGF) also induces TH in the PC-G2 cell line. Although EFG has been shown to be mitogenic for many cultured cells, no neuronal function has been previously reported for this protein. The TH response to EGF is elicited in a dose-dependent fashion at concentrations as low as 0.1 ng/ml and is maximal at 10 ng/ml EGF. The maximal response is observed after 3 to 4 d of exposure to 10 ng/ml EGF. The induction by NGF and EGF is inhibited by their respective antisera. Dexamethasone, a synthetic glucocorticoid which we have previously shown modulates the response of PC-G2 cells to NGF, also modulates the TH induction elicited by EGF.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 5375152
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 84:3; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACIDS
ANIMAL CELLS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL CULTURES
CELL DIVISION
CLONE CELLS
CORTICOSTEROIDS
CULTURE MEDIA
DEXAMETHASONE
ENZYME ACTIVITY
ENZYMES
GLUCOCORTICOIDS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
HYDROXYLASE
KETONES
MITOGENS
MITOSIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PREGNANES
PROTEINS
STEROID HORMONES
STEROIDS
TUMOR CELLS
TYROSINE