Transcriptional regulation of the tyrosine hydroxylase gene by glucocorticoid and cyclic AMP
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Glucocorticoid and cyclic AMP increase tyrosine hydroxylase (TH) activity and mRNA levels in pheochromocytoma cultures. The transcriptional activity of the TH gene, as measured by nuclear run-on assay, is also increased when cultures are treated with the synthetic glucocorticoid dexamethasone or agents that increase intracellular cyclic AMP, such as forskolin and 8-BrcAMP. Both inducers effect transcriptional changes within 10 min after treatment and are maximal after 30 min for forskolin and after 60 min for dexamethasone. The 5' flanking sequences of the TH gene were fused to the bacterial gene chloramphenicol acetyltransferase (CAT), and the hybrid gene was transfected into pheochromocytoma cultures and GH/sub 4/ pituitary cells. In both cell lines, a region of the TH gene containing bases -272 to +27 conferred induction of CAT by cyclic AMP, but not by glucocorticoid. The same results were found when a region of the TH gene containing -773 to + 27 was used. Thus, the sequences required for induction of TH by cyclic AMP are contained within 272 bases of 5' flanking sequence, but sequences sufficient for glucocorticoid regulation are not contained with 773 bases.
- Research Organization:
- Tufts Univ. School of Medicine, Boston, MA
- OSTI ID:
- 5445538
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:11; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMP
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENETIC MAPPING
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
HYDROXYLASES
ISOTOPES
KETONES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
RADIOISOTOPES
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMP
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENETIC MAPPING
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
HYDROXYLASES
ISOTOPES
KETONES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
RADIOISOTOPES
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS