Location and characterization of two widely separated glucocorticoid response elements in the phosphoenolpyruvate carboxykinase gene

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.1.96· OSTI ID:6924578

Petersen, D D; Magnuson, M A; Granner, D K

Chimeric genes were constructed by fusion of various regions of the 5'-flanking sequence from the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene to the chloramphenicol acetyltransferase-coding sequence and to simian virus 40 splice and polyadenylation sequences. These were used to demonstrate that two glucocorticoid regulatory elements (GREs) combine to confer glucocorticoid responsiveness upon the PEPCK gene in H4IIE hepatoma cells. Both elements, distal one whose 5' boundary is located between -1264 and -1111 base pairs and a proximal one located between -468 and -420 base pairs relative to the transcription initiation site, act independently, in various positions and orientations, and upon the heterologous thymidine kinase promoter. Each element accounts for half of the maximal response of the chimeric genes. Therefore, two widely separated enhancerlike elements contribute equally to the response of the PEPCK gene to glucocorticoid hormones. Neither of the PEPCK GREs contains the TGTTCT consensus sequence associated with most other GREs.

• Research Organization: Departments of Molecular Physiology and Biophysics (US)
• OSTI ID: 6924578
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADENYLIC ACID
ADRENAL HORMONES
CELL CULTURES
CHIMERAS
CORTICOSTEROIDS
DISEASES
ENZYMES
GENE REGULATION
GENES
GENETIC MAPPING
GLUCOCORTICOIDS
HEPATOMAS
HYBRIDIZATION
HYDROXY COMPOUNDS
KETONES
MAPPING
MICROORGANISMS
MOSAICISM
NEOPLASMS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHOENOLPYRUVATE
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PREGNANES
SIMIAN VIRUS
STEROIDS
TECHNOLOGY ASSESSMENT
TRANSFERASES
VIRUSES