Subcellular site and mechanism of synthesis of disaturated phosphatidylcholine in alveolar type II cell adenomas
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5375057
The site of synthesis of 1,2-disaturated-(diacyl)-sn-glycero-3-phosphocholine (Sat/sub 2/PC) in mouse alveolar type II cell adenomas has been studied by conducting pulse-chase experiments. Isolation of microsomal and lamellar body fractions from adenomas after a 20-min pulse with (methyl-/sup 3/H)choline demonstrates that Sat/sub 2/PC first appears in the microsomal fraction, and after a short lag subsequently appears in the lamellar body fraction. The kinetics of labeling of Sat/sub 2/PC are consistent with the microsomal membranes functioning as the subcellular site of synthesis for this pulmonary surfactant phospholipid. Short term labeling experiments with (9,10-/sup 3/H)palmitate demonstrate that this fatty acid is incorporated into the sn-2 position of Sat/sub 2/PC at a faster rate than its incorporation into the sn-1 position. This finding indicates that the synthesis of Sat/sub 2/PC occurs by a deacylation-reacylation mechanism.
- Research Organization:
- Oak Ridge Associated Universities, TN
- DOE Contract Number:
- EY-76-C-05-0033
- OSTI ID:
- 5375057
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:17; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis of the dipalmitoyl species of diacyl glycerophosphocholine by rabbit alveolar macrophages
Novel mechanism of diglyceride formation: 12-O-tetra-decanoyl-phorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine
Synthesis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine with labeled acyl groups
Journal Article
·
Fri Jan 31 23:00:00 EST 1986
· Lipids; (United States)
·
OSTI ID:5811200
Novel mechanism of diglyceride formation: 12-O-tetra-decanoyl-phorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5202789
Synthesis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine with labeled acyl groups
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Prep. Biochem.; (United States)
·
OSTI ID:6780421
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADENOMAS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CHOLINE
DISEASES
ESTERS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
LUNGS
MAMMALS
MEMBRANES
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
ADENOMAS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CHOLINE
DISEASES
ESTERS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
LUNGS
MAMMALS
MEMBRANES
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES