Novel mechanism of diglyceride formation: 12-O-tetra-decanoyl-phorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5202789

Daniel, L W; Waite, M; Wykle, R L

12-O-Tetradecanoyl-phorbol-13-acetate (TPA) treatment of Madin-Darby canine kidney (MDCK) cells resulted in an increased incorporation of /sup 32/P/sub i/ and (methyl-/sup 3/H)choline into choline-containing phosphoglycerides (PC). In pulse-chase experiments, TPA treatment caused an increased release of (methyl-/sup 3/H)choline from the PC fraction of prelabeled cells. When cells were prelabeled with (/sup 3/H)arachidonic acid and (/sup 14/C)palmitic acid, TPA treatment resulted in an increased synthesis of /sup 3/H- and /sup 14/C-diglycerides. Further studies were done to determine the relationship between PC breakdown and diglyceride synthesis. Cells were preincubated with ether-linked 1-O-(/sup 3/H)hexadecyl-2-lyso-sn-glycero-3-phosphocholine which was acylated to form 1-O-(/sup 3/H)hexadecyl-2-acyl-sn-glycero-3-phosphocholine. Subsequent treatment of these cells with TPA resulted in an increased synthesis of 1-O-(/sup 3/H)hexadecyl-2-acyl-sn-glycerol compared to that of cells not stimulated with TPA. These findings demonstrate that TPA stimulates PC turnover in MDCK cells and provides evidence for a novel mechanism of diglyceride formation by degradation of PC by a phospholipase C.

• Research Organization: Bowman Gray School of Medicine, Winston-Salem, NC
• OSTI ID: 5202789
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARACHIDONIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CATS
CHOLINE
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERASES
ESTERS
HEXADECANOIC ACID
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASE
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES