Novel mechanism of diglyceride formation: 12-O-tetra-decanoyl-phorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine
12-O-Tetradecanoyl-phorbol-13-acetate (TPA) treatment of Madin-Darby canine kidney (MDCK) cells resulted in an increased incorporation of /sup 32/P/sub i/ and (methyl-/sup 3/H)choline into choline-containing phosphoglycerides (PC). In pulse-chase experiments, TPA treatment caused an increased release of (methyl-/sup 3/H)choline from the PC fraction of prelabeled cells. When cells were prelabeled with (/sup 3/H)arachidonic acid and (/sup 14/C)palmitic acid, TPA treatment resulted in an increased synthesis of /sup 3/H- and /sup 14/C-diglycerides. Further studies were done to determine the relationship between PC breakdown and diglyceride synthesis. Cells were preincubated with ether-linked 1-O-(/sup 3/H)hexadecyl-2-lyso-sn-glycero-3-phosphocholine which was acylated to form 1-O-(/sup 3/H)hexadecyl-2-acyl-sn-glycero-3-phosphocholine. Subsequent treatment of these cells with TPA resulted in an increased synthesis of 1-O-(/sup 3/H)hexadecyl-2-acyl-sn-glycerol compared to that of cells not stimulated with TPA. These findings demonstrate that TPA stimulates PC turnover in MDCK cells and provides evidence for a novel mechanism of diglyceride formation by degradation of PC by a phospholipase C.
- Research Organization:
- Bowman Gray School of Medicine, Winston-Salem, NC
- OSTI ID:
- 5202789
- Report Number(s):
- CONF-8606151-; TRN: 86-031635
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LIPIDS
BIOSYNTHESIS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ARACHIDONIC ACID
CARBON 14 COMPOUNDS
CATS
CHOLINE
HEXADECANOIC ACID
KIDNEYS
LIPASE
PHOSPHORUS 32
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
SYNTHESIS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques