Semi-synthetic preparation of 1-O-(1'-/sup 14/C)hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures

Journal Article · Mon Apr 01 04:00:00 EST 1985 · J. Lipid Res.; (United States)

OSTI ID:5253648

Weber, N; Mangold, H K

Incubation of photomixotrophic cell suspension cultures of rape (Brassica napus) and heterotrophic cell suspension cultures of soya (Glycine max) with 1-O-(1'-/sup 14/C)hexadecyl-sn-glycerol or rac-1-O-(1'-/sup 14/C)hexadecylglycerol leads in high yield (up to 78%) to labeled 1-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. Alkaline hydrolysis of the choline glycerophospholipids yields pure 1-O-(1'-/sup 14/C)hexadecyl-sn-glycero-3-phosphocholine. 1-O-(1'-14C)Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) is obtained by acetylating the lyso compound. The semi-synthetic preparation described leads to labeled platelet activating factor in an overall yield of 50-60% without loss of specific activity.

• Research Organization: H. P. Kaufmann Institut, Muenster, Germany, F.R.
• OSTI ID: 5253648
• Journal Information: J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 4; ISSN JLPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE HYDROLYSIS
AMINES
AMMONIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
BRASSICA
CARBON 14 COMPOUNDS
CELL CULTURES
CHEMICAL REACTIONS
CHOLINE
DECOMPOSITION
DRUGS
FOOD
GLYCEROL
GLYCINE HISPIDA
HYDROLYSIS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
LABELLED COMPOUNDS
LEGUMINOSAE
LIPOTROPIC FACTORS
LYSIS
ORGANIC COMPOUNDS
PLANTS
QUATERNARY COMPOUNDS
REACTION KINETICS
SOLVOLYSIS
TRACER TECHNIQUES
VEGETABLES
YIELDS