Metabolism of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine by cell cultures

Journal Article · Mon Dec 20 04:00:00 UTC 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90680-4· OSTI ID:6149285

Cabot, M C; Blank, M L; Welsh, C J; Horan, M J; Cress, E A; Snyder, F

The metabolism of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) was studied using various cultured cell lines. All incubations were done in the presence of bovine serum albumin and serum-free media, since albumin eliminates the adsorption of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine to cultureware and serum enzymes interfere. Human leukemia (HL-60) cells, MDCK canine kidney cells, and transformed and nontransformed clones of mouse C3H1OT1/2 cells display varying rates of uptake, degradation, and capacities for reacylation of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine. HL-60 cells displayed the highest uptake rate (24.6 pmol/mg cell protein/15 min). Whereas C3H1OT1/2 cells in culture showed uptake rates comparable to other cells tested, they displayed a relative metabolic inertness towards 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine.

• Research Organization: Oak Ridge Associated Univ., Tennessee
• OSTI ID: 6149285
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:25; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
BODY
CELL CULTURES
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
ESTERS
FIBROBLASTS
KIDNEYS
LIPIDS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
SOMATIC CELLS
TUMOR CELLS
UPTAKE